GREGORIUS GERIK Pengarang Dr. Theresia N. I. Koni, S.Pt, M.Si Dosen Pembimbing 2 Dr. Radempta Wea, S.Pt., MP Dosen Pembimbing 1 Dr. Cytske sabuna, S.Pt., M.Si Penguji 1 Aholiab Aoetpah, S. Pt., M.Rur., Sc., Ph.D Penguji 2 Dr. Theresia N. I. Koni, S. Pt, M.Si Penguji 2 Dr. Redempta Wea, S. Pt., MP Penguji 1 AHMAD FADIL Contributor

Peanut shell waste is one of the wastes that can be used as feedstuff. This study aims to examine the effect of different levels of goat rumen fluid on the content of peanut shell fiber fraction. The research was conducted at the General Laboratory of the Kupang State Agricultural Polytechnic from April to September 2020.The study used a completely randomized design consisting of 4 treatments and 5 replications, namely; R0: peanut shell waste without goat rumen fluid, R1: peanut shell waste uses 25% goat rumen fluid, R2: peanut shell waste uses 30% goat rumen fluid, R3: peanut shell waste uses 35% goat rumen fluid. Data were analyzed using Analysis of Variance (ANOVA) and further tests with the Duncan Multiple Range Test method.The variables observed were fiber fraction (NDF, ADF, Hemicellulose, Cellulose, and Lignin).The results showed that goat rumen fluid had no significant effect (P> 0.05) on NDF levels with an average of 86.88, ADF had a significant effect (P <0.05) with an average of 79.01, hemicellulose had no significant effect. (P> 0.05) with an average of 7.87, cellulose had no significant effect (P> 0.05) with a mean of 43.40, and lignin had no significant effect (P> 0.05) with an average 35.03. Keywords: Peanut Shell Waste, Fermentation, Fiber Fraction, Goat Rumen Fluid